The concentration of Protein in solution can be determined by substituting the molecular weight, extinction coefficient and λ max into a derived form of the Beer - Lambert Law. A substance's λ max is the wavelength at which it experiences the strongest absorbance. For Protein, this wavelength is nm. The absorbance at λ max can be measured using a spectrophotometer.
10. How to calculate number of amino acid residue in protein from its molecular weight
Step 3: Calculate number of moles in g of hydrogen chloride using following formula. Number of moles: weight (in grams)/molar mass (or molecular weight) Number of moles in g of hydrogen chloride = / = moles. It means moles of hydrogen chloride is present in ml of hydrochloric acid solution. The molecular weight (mw) of an oligopeptide or a protein can be determined by summation of the mw of its corresponding amino acid sequence. mass of protein (g) = moles protein (mol) x molecular weight of protein (Da) For the purposes of molarity calculations, the Da unit is equivalent to g/mol. Peptide to Molecular Weight molecular weight of protein (Da) = length of peptide (aa) x (g/aa), where is the average MW of an amino acid weighted by frequency of occurence.
Tool to calculate molecular weight of protein via DNA or amino acid sequence. May 13, · Molecular Weight Molecular Weight Scan Mass Spectrometry Result: Target Mass. All Trypsin Chymotrypsin V8 Protease. Percent Difference Maximum Difference (Daltons) Min Max: Mass Range (Daltons) Calculate masses of b+ and y+ daughter ions. Use Monoisotopic Masses (Not Isotopic Averages) Methionine to Selenomethionine Cysteine to .